Please find below a list of protocols that have relevance to researchers working on topics related to those that are the focus of the INDEPTH network.
An important Objective of this COST Action is to provide access to protocols that have relevance to INDEPTH researchers.
> These protocols have been written by experts from within INDEPTH. This means that they have been actually tested and used in the lab by real experts!
> These protocols have either featured within INDEPTH Training Schools or have been published as Methods papers within peer-reviewed journals.
Detailed protocols developed as part of the INDEPTH Academy
Protocol for Hi-C Chromosome Confirmation Capture. This method is adapted from research in the labs of Chang Liu and Silin Zhong.
Protocol for Single Molecular Fluorescent In Situ Hybridisation. This method is adapted from research in Stefanie Rosa’s lab and provides instructions for this powerful technique for analysis of gene expression
Protocol for Localising Total mRNA in Plant Cells . This method is adapted by Geraint Parry for a selection of published methodologies.
Protocol for Fluorescence Activated Nuclear Sorting (FANS) for RNA extraction, DNA extraction or Chromatin Immunoprecipitation. This method is adapted from protocols developed in Ortrun Mittelsten Scheid’s lab.