Quantitative imaging and analysis of the plant nucleus in 3D
A major bottleneck towards a better understanding of nuclear organization and function in plants is the lack of dedicated imaging and analysis procedures that meet the challenges of plant cells. WG1 aims to coordinate efforts to improve sample preparation, image acquisition and analysis approaches that meet the specific challenges of plants and will allow imaging plant nuclei in their tissue context as part of a living organism.
Workgroup 1 has established a free-to-access Image Repository hosted by OMERO at Florida State University through a collaboration with Professor Hank Bass.
Members of WG1:
- Phillippe Andrey, INRA Centre de Versailles-Grignon, France
- Zikrija Avdagic, University of Sarajevo, Bosnia and Herzegovina
- Phillippe Baert, Nikon, Belgium
- Célia Baroux, University of Zürich, Switzerland
- Hank Bass, Florida State University, United States
- Elise Bertrand, Zeiss, Germany
- Emir Buza, University of Sarajevo, Bosnia and Herzegovina
- Winnok De Vos, University of Antwerp, Belgium
- Jaroslav Dolezel, Institute of Experimental Botany, Czech Republic
- Susan Duncan, Earlham Institute, UK
- David Evans, Oxford Brookes University, UK
- Paul Fransz, University of Amsterdam, Netherlands
- Andy French, University of Nottingham, UK
- Valérie Gaudin, INRA Centre de Versailles-Grignon, France
- Katja Graumann, Oxford Brookes University, UK
- Thorsten Hamann, Norwegian University of Science and Technology, Norway
- Mathieu Ingouff, IRD, France
- Pauline Jullein, University of Bern, Switzerland
- Patrik Kamencay, University of Zilina, Slovakia
- Chang Liu, Center for Molecular Biology of Plants, Germany
- Martin Lysak, CEITEC, Czech Republic
- Peter Majer, Bitplane, Switzerland
- Kristine Margaryan, Yerevan State University, Armenia
- Sebastian Marquardt, University of Copenhagen
- Iris Meier, The Ohio State University, United States
- Ortrun Mittelsten Scheid, Gregor Mendel Institute of Molecular Plant Biology, Austria
- Anna Nebish, Yerevan State University, Armenia
- Samir Omanovic, University of Sarajevo, Bosnia and Herzegovina
- Ales Pecinka, Institute of Experimental Botany, Czech Republic
- Beáta Petrovska, Centre of New Technologies University of Warsaw, Poland
- Dariusz Plewczynski, Institute of Experimental Botany, Czech Republic
- Frédéric Pontvianne, Laboratoire Génome et Développement des Plantes, France
- Dimiter Prodanov, Interuniversity Microelectronics Center, Belgium
- Stefanie Rosa, Swedish University of Agricultural Sciences, Sweden
- Anne-Catherine Schmit, Institut de Biologie Moléculaire des plantes, France
- Kentaro Tamura, Kyoto University, Japan
- Christophe Tatout, Genetics, Reproduction and Development, France
- Ahmet Tek, Niğde Ömer Halisdemir Üniversitesi, Turkey
- Imre Vass, Biological Research Centre, Institute of Plant Biology – Laboratory of Molecular Stress an Photobiology, Hungary
Recent publications from members of WG1:
Bilalovic, O, Avdagic, Z.; Omanovic, S, Besic, I, Letic, V, Tatout, C (2020) Mathematical Modelling of Ground Truth Image for 3D Microscopic Objects Using Cascade of Convolutional Neural Networks Optimized with Parameters’ Combinations Generators. Symmetry, 12, 416 https://www.mdpi.com/2073-8994/12/3/416
Biel AM, Moser M, Meier I (2019) A role for plant KASH proteins in regulating stomatal dynamics. Plant Physiol. doi: 10.1104/pp.19.01010
Le Goff S, Nur Keçeli B, Jerabkova H, Heckmann S, Rutten T, Cotterell S, Schubert V, Roitinger E, Mechtler K, Franklin FCH, Tatout C, Houben A, Geelen D, Probst AV, Lermontova I (2019) The H3 histone chaperone NASPSIM3 escorts CenH3 in Arabidopsis. Plant J doi: 10.1111/tpj.14518
Dumur T, Duncan S, Graumann K, Desset S, Randall RS, Scheid OM, Prodanov D, Tatout C, Baroux C (2019) Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions. Nucleus. 181-212. doi: 10.1080/19491034.2019.1644592
Rutowicz K, Lirski M, Mermaz B, Teano G, Schubert J, Mestiri I, Kroteń MA, Fabrice TN, Fritz S, Grob S, Ringli C, Cherkezyan L, Barneche F, Jerzmanowski A, Baroux C (2019) Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 20(1):157. doi: 10.1186/s13059-019-1767-3
Goto C, Hashizume S, Fukao Y, Hara-Nishimura I, Tamura K (2019) Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction. Nucleus 81-92. doi: 10.1080/19491034.2019.1603093
Pradillo M, Evans D and Graumann K (2019) The nuclear envelope in higher plant mitosis and meiosis. Nucleus DOI: 10.1080/19491034.2019.1587277
Gumber HK, McKenna JF, Estrada AL, Tolmie AF, Graumann K, Bass HW (2019) Identification and characterization of genes encoding the nuclear envelope LINCcomplex in the monocot species Zea mays. J Cell Sci pii: jcs.221390. doi: 10.1242/jcs.221390
Newman-Griffis AH, Del Cerro P, Charpentier M, Meier I (2018) Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis. Plant Physiol. doi: 10.1104/pp.18.01111
Poulet A, Zhou X, Tamura K, Meier I, Tatout C, Graumann K, Evans, DE (2018) Computational Methods for Studying the Plant Nucleus in Gregg G Gundersen and Howard J Worman (ed.), The LINC Complex (Methods in Molecular Biology, Vol. 1840). ISBN: 9781493986903 eISBN: 9781493986910
Groves NR, Biel AM, Newman-Griffis AH, Meier I (2018) Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals. Plant Physiol. 176(1):230-241. doi: 10.1104/pp.17.00788.
Voisin M, Vanrobays E, Tatout C (2018) Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System. Methods Mol Biol. 1840:221-235. doi: 10.1007/978-1-4939-8691-0_16
